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Developmental Therapeutics Program (DTP)
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A kinase (PRKA) anchor protein 1 RNA [primary]

Primary Reference(s):

A kinase (PRKA) anchor protein 1 RNA

and

A kinase (PRKA) anchor protein 1 RNA

Experiment Id: 10619

Pattern Id: GC10315

Clone number: 510182

GenBank Acc: AA053135

Rationale: Relative RNA levels for nearly 10,000 human clones were measured in a microarray experiment. Some of these clones have been linked to known genes (based on clustering information from Unigene) - follow the links to Unigene and GeneCards, where information on possible function can be found. Other clones represent as yet unnamed genes - follow the Unigene link for more information. All gene assignments should be considered tentative. Sequence information for a clone can be found through GenBank or Unigene, and can be used to search sequence databases.

Investigator(s):

Botstein, Dr. David

Stanford University School of Medicine


Weinstein, Dr. John

National Cancer Institute

Method: Microarray

Unit: log(mRNA levels in cell line/mRNA levels in reference pool)

Protocol: mRNA was isolated from logarithmically growing cells. Labelled cDNA was prepared by reverse transcription of test cell mRNA in the presence of Cy5-dUTP. Reference probes were made by pooling equal amounts of mRNA from HL-60, K562, NCI-H226, COLO205, SNB-19, LOX-IMVI, OVCAR-3, OVCAR-4, CAKI-1, PC-3, MCF7 and Hs578T cell lines. Labelled cDNA was prepared from the pooled mRNA by reverse transcription in the presence of Cy3-dUTP. Test and reference probes were combined, denatured and hybridized overnight to Synteni microarrays containing cDNA from 9703 human clones. Arrays were scanned using a laser-scanning microscope. The ScanAlyze program was used to analyze the microarray images and prepare data files for further analysis.

Target Data:

Compare:

Unigene

An experimental system for automatically partitioning GenBank sequences into a non-redundant set of gene-oriented clusters.

Gene

Presents information on official nomenclature, aliases, sequence accession numbers, phenotypes, EC numbers, MIM numbers, UniGene clusters, map information, Gene Ontology annotations, and relevant web sites. Replaces LocusLink.

OMIM

A catalog of human genes and genetic disorders.

PubMed

Search PubMed for articles on:

CGAP

CGAP provides information on gene expression, SNPs, functional pathways and more in normal, precancerous and cancer cells.

Email questions concerning DTP's molecular targets program to: [Moltarget@mail.nih.gov]

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