S-100 protein PRO [primary]
Experiment Id: 11570 Pattern Id: MT1278 Clone number: |
Rationale: The solid tumor lines were characterized individually using immunocytochemical techniques to determine their relative degrees of representativity for the subpanels within which they are grouped. |
Investigator(s): Stinson, Dr. Sherman NCI-FCRDC |
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Method: Immunostaining Unit: Percent positive cells Protocol: Cells were grown to near-confluence and harvested with a cell scaper. Cytology specimens were propared on glass or Teflon-coated slides. Immunostaining was performed on air-dired, unfixed specimens (for cell surface antigens) or on actone fixed specimens (for intracellular antigens) with commercially available kits. Slides were prepared froma minimum of 2 separate cultures for each cell line. Micorscopically, 3 separate fields, each with ~100 cells, were selected from each slide and the % positive stained cells was counted. Results from all fields and slides were averaged and rounded to the nearest 5%. |
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An experimental system for automatically partitioning GenBank sequences into a non-redundant set of gene-oriented clusters. |
Presents information on official nomenclature, aliases, sequence accession numbers, phenotypes, EC numbers, MIM numbers, UniGene clusters, map information, Gene Ontology annotations, and relevant web sites. Replaces LocusLink. |
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A catalog of human genes and genetic disorders. |
Search PubMed for articles on: |
CGAP provides information on gene expression, SNPs, functional pathways and more in normal, precancerous and cancer cells. |
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Email questions concerning DTP's molecular targets program to: [Moltarget@mail.nih.gov]