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Developmental Therapeutics Program (DTP)
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S-100 protein PRO [primary]

Primary Reference(s):

S-100 protein PRO

Experiment Id: 11570

Pattern Id: MT1278

Clone number:

GenBank Acc:

Rationale: The solid tumor lines were characterized individually using immunocytochemical techniques to determine their relative degrees of representativity for the subpanels within which they are grouped.

Investigator(s):

Stinson, Dr. Sherman

NCI-FCRDC

Method: Immunostaining

Unit: Percent positive cells

Protocol: Cells were grown to near-confluence and harvested with a cell scaper. Cytology specimens were propared on glass or Teflon-coated slides. Immunostaining was performed on air-dired, unfixed specimens (for cell surface antigens) or on actone fixed specimens (for intracellular antigens) with commercially available kits. Slides were prepared froma minimum of 2 separate cultures for each cell line. Micorscopically, 3 separate fields, each with ~100 cells, were selected from each slide and the % positive stained cells was counted. Results from all fields and slides were averaged and rounded to the nearest 5%.

Target Data:

Compare:

Unigene

An experimental system for automatically partitioning GenBank sequences into a non-redundant set of gene-oriented clusters.

Gene

Presents information on official nomenclature, aliases, sequence accession numbers, phenotypes, EC numbers, MIM numbers, UniGene clusters, map information, Gene Ontology annotations, and relevant web sites. Replaces LocusLink.

OMIM

A catalog of human genes and genetic disorders.

PubMed

Search PubMed for articles on:

CGAP

CGAP provides information on gene expression, SNPs, functional pathways and more in normal, precancerous and cancer cells.

Email questions concerning DTP's molecular targets program to: [Moltarget@mail.nih.gov]

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