Experiment Id: 19339

Pattern Id: MT1333

Clone number:

GenBank Acc:

Rationale: Beta 2-microglobulin, as identified by the LMR-12 antibody, is upregulated in multidrug-resistant cells.

Investigator(s):

Shoemaker, Dr. Robert

National Cancer Institute

Method: Immunocytochemistry

Unit: Staining index

Protocol: Cytocentrifuge preparations of tumor cell lines were air-dried and fixed in acetone. Slides were incubated with LMR-12 or isotype matched rat Ig, then with biotinylated rabbit anti-rat IgG or anti-mouse IgG and streptavidin-HRP. Color development was evaluated on coded slides to avoid bias in scoring. A semiquantitative 'staining index' was calculated as the product of the fraction of positive cells and the average staining intensity estimated on a scale from 0 (negative) to 3 (very strong positive). At least three tests for each cell line were used for calculation of the average staining index.

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