Experiment Id: 371663

Pattern Id: MT15750

Clone number:

GenBank Acc:

Rationale: Metabolic profiling can provide information on the physiological state of a cell line.

Investigator(s):

Mohney, Dr. Robert

Metabolon, Inc.

Method: LC/MS and GC/MS

Unit: Relative units

Protocol: Triplicate samples (most cell lines) were prepared with a proprietary series of organic and aqueous extractions to remove the protein fraction while allowing maximum recovery of small molecules. Samples were split and analyzed by LC/MS and GC/MS. Compounds were identified by comparison to library entries of purified standards or recurrent unknown entities. Data was normalized to correct variation resulting from instrument inter-day tuning differences.

Target Data:

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Unigene

An experimental system for automatically partitioning GenBank sequences into a non-redundant set of gene-oriented clusters.

Gene

Presents information on official nomenclature, aliases, sequence accession numbers, phenotypes, EC numbers, MIM numbers, UniGene clusters, map information, Gene Ontology annotations, and relevant web sites. Replaces LocusLink.

OMIM

A catalog of human genes and genetic disorders.

PubMed

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CGAP

CGAP provides information on gene expression, SNPs, functional pathways and more in normal, precancerous and cancer cells.