E-cadherin promoter methylation - site 19 DNAme [primary]
Experiment Id: 359100 Pattern Id: MT3180 Clone number: |
Rationale: Methylation of the E-cadherin promoter may affect gene expression. |
Investigator(s): Weinstein, Dr. John National Cancer Institute |
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Method: Bisulfite/Sequencing Unit: % methylation Protocol: Genomic DNA (5 ?g) from each cell line was treated with sodium bisulfite at 50?C for 17 h using the CpGenome DNA Modification kit from Chemicon International (Temecula, CA) according to the instructions of the manufacturer (except for a 5x volume scale-up through the first washing step on day 2). The DNA was then resuspended in 125 ?L of 10 mmol/L Tris with 1 mmol/L of EDTA (pH 7.4; K-D Medical, Columbia, MD). The protocol produced enough material for ~100 PCR sequencing reactions. |
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Presents information on official nomenclature, aliases, sequence accession numbers, phenotypes, EC numbers, MIM numbers, UniGene clusters, map information, Gene Ontology annotations, and relevant web sites. Replaces LocusLink. |
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