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E-cadherin promoter methylation - site 22 DNAme [primary]

Primary Reference(s):

E-cadherin promoter methylation - site 22 DNAme

Experiment Id: 359104

Pattern Id: MT3184

Clone number:

GenBank Acc:

Rationale: Methylation of the E-cadherin promoter may affect gene expression.

Investigator(s):

Weinstein, Dr. John

National Cancer Institute

Method: Bisulfite/Sequencing

Unit: % methylation

Protocol: Genomic DNA (5 ?g) from each cell line was treated with sodium bisulfite at 50?C for 17 h using the CpGenome DNA Modification kit from Chemicon International (Temecula, CA) according to the instructions of the manufacturer (except for a 5x volume scale-up through the first washing step on day 2). The DNA was then resuspended in 125 ?L of 10 mmol/L Tris with 1 mmol/L of EDTA (pH 7.4; K-D Medical, Columbia, MD). The protocol produced enough material for ~100 PCR sequencing reactions.

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Unigene

An experimental system for automatically partitioning GenBank sequences into a non-redundant set of gene-oriented clusters.

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Presents information on official nomenclature, aliases, sequence accession numbers, phenotypes, EC numbers, MIM numbers, UniGene clusters, map information, Gene Ontology annotations, and relevant web sites. Replaces LocusLink.

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A catalog of human genes and genetic disorders.

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