E-cadherin promoter methylation - site 22 DNAme [primary]
Experiment Id: 359104 Pattern Id: MT3184 Clone number: |
Rationale: Methylation of the E-cadherin promoter may affect gene expression. |
Investigator(s): Weinstein, Dr. John National Cancer Institute |
|
|
Method: Bisulfite/Sequencing Unit: % methylation Protocol: Genomic DNA (5 ?g) from each cell line was treated with sodium bisulfite at 50?C for 17 h using the CpGenome DNA Modification kit from Chemicon International (Temecula, CA) according to the instructions of the manufacturer (except for a 5x volume scale-up through the first washing step on day 2). The DNA was then resuspended in 125 ?L of 10 mmol/L Tris with 1 mmol/L of EDTA (pH 7.4; K-D Medical, Columbia, MD). The protocol produced enough material for ~100 PCR sequencing reactions. |
Target Data:
|
Compare:
|
|
|
An experimental system for automatically partitioning GenBank sequences into a non-redundant set of gene-oriented clusters. |
Presents information on official nomenclature, aliases, sequence accession numbers, phenotypes, EC numbers, MIM numbers, UniGene clusters, map information, Gene Ontology annotations, and relevant web sites. Replaces LocusLink. |
||
|
A catalog of human genes and genetic disorders. |
Search PubMed for articles on: |
CGAP provides information on gene expression, SNPs, functional pathways and more in normal, precancerous and cancer cells. |
|
Email questions concerning DTP's molecular targets program to: [Moltarget@mail.nih.gov]