Methylation of CpG site(s) -- 6 -- in JAK2 DNAme [primary]
Experiment Id: 362471 Pattern Id: MT6557 Clone number: |
Rationale: DNA methylation can affect gene expression. |
Investigator(s): Ehrich, Dr. Mathias SEQUENOM, Inc. |
|
|
Method: Bisulfite PCR MALDI-TOF Unit: Fraction of DNA methylated Protocol: DNA methylation by gene-specific amplification of bisulfite-treated DNA followed by in vitro transcription and MALDI-TOF analysis. The amplification regions were designed to cover CpG islands (CGIs) overlapping the 5' UTR of genes. If no CGI nearby, the sequence directly surrounding the 5' UTR was used for primer design, and if no CpGs were in this region, the next upstream flanking CGI in a 10-kb window was used. |
Target Data:
|
Compare:
|
|
|
An experimental system for automatically partitioning GenBank sequences into a non-redundant set of gene-oriented clusters. |
Presents information on official nomenclature, aliases, sequence accession numbers, phenotypes, EC numbers, MIM numbers, UniGene clusters, map information, Gene Ontology annotations, and relevant web sites. Replaces LocusLink. |
||
|
A catalog of human genes and genetic disorders. |
Search PubMed for articles on: |
CGAP provides information on gene expression, SNPs, functional pathways and more in normal, precancerous and cancer cells. |
|
Email questions concerning DTP's molecular targets program to: [Moltarget@mail.nih.gov]