DTP Branches and Offices
Last Updated: 05/08/15
Cell Lines in the In Vitro Screen
Note: This is a list of the 60 human cancer cell lines used in the screen and maintained at NCI-Frederick. Additional lines evaluated for use in the screen and currently available are listed separately at the bottom of the page.
Please note the links for more information on the SNB-19, U251, NCI/ADR-RES, and MDA-MB-435 cell lines.
| Cell Line Name | Panel Name | Doubling Time | Inoculation Density |
|---|---|---|---|
| CCRF-CEM | Leukemia | 26.7 | 40000 |
| HL-60(TB) | Leukemia | 28.6 | 40000 |
| K-562 | Leukemia | 19.6 | 5000 |
| MOLT-4 | Leukemia | 27.9 | 30000 |
| RPMI-8226 | Leukemia | 33.5 | 20000 |
| SR | Leukemia | 28.7 | 20000 |
| A549/ATCC | Non-Small Cell Lung | 22.9 | 7500 |
| EKVX | Non-Small Cell Lung | 43.6 | 20000 |
| HOP-62 | Non-Small Cell Lung | 39 | 10000 |
| HOP-92 | Non-Small Cell Lung | 79.5 | 20000 |
| NCI-H226 | Non-Small Cell Lung | 61 | 20000 |
| NCI-H23 | Non-Small Cell Lung | 33.4 | 20000 |
| NCI-H322M | Non-Small Cell Lung | 35.3 | 20000 |
| NCI-H460 | Non-Small Cell Lung | 17.8 | 7500 |
| NCI-H522 | Non-Small Cell Lung | 38.2 | 20000 |
| COLO 205 | Colon | 23.8 | 15000 |
| HCC-2998 | Colon | 31.5 | 15000 |
| HCT-116 | Colon | 17.4 | 5000 |
| HCT-15 | Colon | 20.6 | 10000 |
| HT29 | Colon | 19.5 | 5000 |
| KM12 | Colon | 23.7 | 15000 |
| SW-620 | Colon | 20.4 | 10000 |
| SF-268 | CNS | 33.1 | 15000 |
| SF-295 | CNS | 29.5 | 10000 |
| SF-539 | CNS | 35.4 | 15000 |
| SNB-19 | CNS (For more Information) |
34.6 | 15000 |
| SNB-75 | CNS | 62.8 | 20000 |
| U251 | CNS (For more information) |
23.8 | 7500 |
| LOX IMVI | Melanoma | 20.5 | 7500 |
| MALME-3M | Melanoma | 46.2 | 20000 |
| M14 | Melanoma | 26.3 | 15000 |
| MDA-MB-435 | Melanoma (For more information) |
25.8 | 15000 |
| SK-MEL-2 | Melanoma | 45.5 | 20000 |
| SK-MEL-28 | Melanoma | 35.1 | 10000 |
| SK-MEL-5 | Melanoma | 25.2 | 10000 |
| UACC-257 | Melanoma | 38.5 | 20000 |
| UACC-62 | Melanoma | 31.3 | 10000 |
| IGR-OV1 | Ovarian | 31 | 10000 |
| OVCAR-3 | Ovarian | 34.7 | 10000 |
| OVCAR-4 | Ovarian | 41.4 | 15000 |
| OVCAR-5 | Ovarian | 48.8 | 20000 |
| OVCAR-8 | Ovarian | 26.1 | 10000 |
| NCI/ADR-RES | Ovarian (For more information) |
34 | 15000 |
| SK-OV-3 | Ovarian | 48.7 | 20000 |
| 786-0 | Renal | 22.4 | 10000 |
| A498 | Renal | 66.8 | 25000 |
| ACHN | Renal | 27.5 | 10000 |
| CAKI-1 | Renal | 39 | 10000 |
| RXF 393 | Renal | 62.9 | 15000 |
| SN12C | Renal | 29.5 | 15000 |
| TK-10 | Renal | 51.3 | 15000 |
| UO-31 | Renal | 41.7 | 15000 |
| PC-3 | Prostate | 27.1 | 7500 |
| DU-145 | Prostate | 32.3 | 10000 |
| MCF7 | Breast | 25.4 | 10000 |
| MDA-MB-231/ATCC | Breast | 41.9 | 20000 |
| MDA-MB-468 | Breast | 62 | 2000 |
| HS 578T | Breast | 53.8 | 20000 |
| MDA-N Not Available |
Breast | 22.5 | 15000 |
| BT-549 | Breast | 53.9 | 20000 |
| T-47D | Breast | 45.5 | 20000 |
Additional Lines
| Cell Line Name | Panel Name | Doubling Time | Inoculation Density |
|---|---|---|---|
| LXFL 529 | Non-Small Cell Lung | 10000 | |
| DMS 114 | Small Cell Lung | 20000 | |
| SHP-77 | Small Cell Lung | 40000 | |
| DLD-1 | Colon | 50000 | |
| KM20L2 | Colon | 10000 | |
| SNB-78 | CNS | 20000 | |
| XF 498 | CNS | 20000 | |
| RPMI-7951 | Melanoma | 20000 | |
| M19-MEL | Melanoma | 10000 | |
| RXF-631 | Renal | 10000 | |
| SN12K1 | Renal | 10000 | |
| P388 | Leukemia | 50000 | |
| P388/ADR | Leukemia | 50000 |
NCI-60 Human Tumor Cell Lines Screen
- Main
- Announcement: Update to NCI-60 (August 2023)
- Compound Submission
- Data Retrieval and Testing Decisions
- List of NCI-60 Human Tumor Cell Lines
- Screening methodology (One-dose and Five-dose Assay)
- Sample Handling and Preparation
- Database of Screening Results
- Molecular Characterization of NCI-60
- Publications