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Last Updated: 03/26/19

The NCI Program for Natural Product Discovery (NPNPD) Prefractionated Library

The NCI Program for Natural Product Discovery (NPNPD) Prefractionated Library

What is the NPNPD prefractionated library?

  • Publicly accessible, free of charge, and open to screening against any disease target
  • Launched in January 2019, 150,000 natural product fractions are available; additional fraction sets added each consecutive year until at least 1,000,000 fractions are available

What are the aims of the NPNPD?

  • Reinvigorate natural product-based research
  • Prefractionate more than 125,000 crude extracts
  • Generate a library of 1,000,000 natural product fractions for modern high-throughput targeted screening technologies
  • Develop integrated analytical resources for rapid isolation and structure elucidation of biologically active natural products

What is the value of the NPNPD library for drug screening?

  • Important sources of new drugs and drug leads
  • The largest and most diverse publicly available collection of natural product extracts and fractions available for high throughput screening

How was the NPNPD prefractionated library generated?

  • A highly-automated solid-phase extraction to separate a crude natural product extract into seven fractions of decreasing polarity
  • Comprised of plant, marine invertebrate, and microbial samples; represents one of the largest sources of chemical diversity available for screening

What is the format of the library for screening?

  • 384-well plates containing 10 µg/well of individual samples (Fractions and crude extracts) dissolved in 2 ÁL of DMSO
  • The first two columns on each plate are empty for use as control wells in screening

Distribution of materials

Access is subject to completing and signing a Material Transfer Agreement.

Request contact:

Carol Haggerty
Natural Products Branch
Developmental Therapeutics Program
Division of Cancer Treatment and Diagnosis
National Cancer Institute
Riverside Five, Suite 400, Room 4101
P. O. Box B
Frederick, MD, 21702

+1.301.624.1269 (Voice)
+1.301.631.3026 (Facsimile)
Email: NCINatProdRep@mail.nih.gov

Requests should be copied to:

Barry R. O’Keefe, Ph.D.
Chief, Natural Products Branch,
Developmental Therapeutics Program
Division of Cancer Treatment and Diagnosis
NCI
Bldg. 562, Rm. 201 Frederick, MD 21702
Tel: (301)-846-5332
Fax: (301)-846-6872
okeefeba@mail.nih.gov

About the Branch Chief

Dr. Barry R. O’Keefe Dr. Barry R. O’Keefe received a B.S. in Botany from Michigan State University, and a Ph.D. in Pharmacognosy from the University of Illinois at Chicago in 1993. In 1994 he joined the National Cancer Institute’s Laboratory of Drug Discovery Research and Development to study novel proteins from natural products extracts. More…